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SurePRIME Core, MP Biomedicals

For high fidelity PCR extremely low error rates optimal blunt end cloning. The most thermostable polymerase available.

  • Chemically modifi ed Taq DNA Polymerase and dNTPs in one kit
  • SurePRIMETM core kits contain all the reagents required for hot start PCR polymerase, high purity dNTPs mix (99%) and optimized incubation buffers
  • Store at –20 °C

Isis is a proofreading DNA polymerase and amplifies DNA with 40 times more accuracy than standard Taq DNA polymerase. The error rate is 0.66×10-6 mutation frequency/bp/duplication as determined by the Flaman(1,2) method. Isis DNA polymerase is isolated from Pyrococcus abyssi and is an excellent choice for applications requiring high fidelity, like error-free cloning. Isis DNA Polymerase does not require primer 3'-end protection, unlike many other proofreading enzymes. This saves time during reaction setup and optimization. Isis DNA polymerase is able to amplify up to 6 kb on genomic DNA templates and 18 kb on phage DNA under a variety of amplification conditions. Isis DNA polymerase offers robust activity and flexibility.



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Description Size Supplier No. VWR Catalog Number Unit Price Quantity
Kit and Assay 5×250 U 11EPHSK105 IC11EPHSK105 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
  • State issued document with your organization's Resale Tax ID Number
  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

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