For high fidelity PCR extremely low error rates optimal blunt end cloning. The most thermostable polymerase available.
- Chemically modifi ed Taq DNA Polymerase and dNTPs in one kit
- SurePRIMETM core kits contain all the reagents required for hot start PCR polymerase, high purity dNTPs mix (99%) and optimized incubation buffers
- Store at –20 °C
Isis is a proofreading DNA polymerase and amplifies DNA with 40 times more accuracy than standard Taq DNA polymerase. The error rate is 0.66×10-6 mutation frequency/bp/duplication as determined by the Flaman(1,2) method. Isis DNA polymerase is isolated from Pyrococcus abyssi and is an excellent choice for applications requiring high fidelity, like error-free cloning. Isis DNA Polymerase does not require primer 3'-end protection, unlike many other proofreading enzymes. This saves time during reaction setup and optimization. Isis DNA polymerase is able to amplify up to 6 kb on genomic DNA templates and 18 kb on phage DNA under a variety of amplification conditions. Isis DNA polymerase offers robust activity and flexibility.