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AMV RTASE, MP Biomedicals

Supplier: MP BIOMEDICALS (FKA ICN BIOMED
11EMAMV200 11EMAMV203
High efficiency enzyme from moloney murine leukemia virus.

  • From avian myeloblastosis virus
  • Withstands temperatures of 55 to 60 °C
  • RNase H and unwinding activity
  • Synthesis of full length copies of cDNA -10 kb
  • Low RNase H activity
  • Recombinant enzyme

AMV Reverse Transcriptase is an RNA-dependent DNA polymerase. The enzyme is composed of two polypeptides. The molecular weight
of the one subunit is 68000 Da and that of the other subunit 92000 Da. It is widely used for first and second strand synthesis of complementary DNA (cDNA) from mRNA templates for cloning or for hybridization probes. Such synthesis requires a primer, commonly oligo(dT) bound to the 3'-poly(A) region of mRNA. Polymerization proceeds in the 5' to 3' direction with no 5' to 3' exonuclease activity. Under the right conditions, high yields of full length cDNA can be obtained using AMV reverse transcriptase. AMV Reverse Transcriptase is suitable for synthesis of cDNA using specific primers, at temperatures of 55 to 60 °C. This high reaction temperature permits transcription of RNA containing secondary structure.




 

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Size Supplier No. VWR Catalog Number Unit Price Quantity
200 U 11EMAMV200 IC11EMAMV200 Each Retrieving

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

3×200 U 11EMAMV203 IC11EMAMV203 Each Retrieving

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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SPECIFICATIONS

Storage Conditions Store at –20 °C.
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