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80% Glycerol Sterile Solution, MP Biomedicals

Supplier: MP BIOMEDICALS (FKA ICN BIOMED
113055034
Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis.

  • Suitable for cell culture, electrophoresis, and insect cell culture
  • Assay is ≥99.5% (GC), corrected for water
  • Easy to pipet; excellent for freezing strains
  • Storage: 0 to 5 °C

Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component. It has been used in purification of bacteriophage λ particles by step gradients, calcium-phosphate-mediated transfection of eukaryotic cells with plasmid DNA, DEAE-dextranmediated transfection of DNA into cells, DNA sequencing reactions, and mediation of mispriming in PCR. The use of glycerol in improved focusing of alkaline pH protein samples in immobilized pH gradient (IPG) strips has been published. Studies of the effect of glycerol on separation of DNA fragments by capillary electrophoresis have been reported.

Sterile 80% Glycerol solution offered is less viscous and more convenient to pipette than 100% glycerol.

Bacteria and yeast cells can be stored for months to years in a liquid or solid form at –10 to –15 °C or frozen at –20 to –80 °C in 20 to 80% glycerol. Glycerol is added to fresh cells from liquid or plate cultures and stored in the freezer. Fresh media cultures are inoculated by dipping into the frozen stock with a sterile loop or pipette.




 

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Description Size Supplier No. VWR Catalog Number Unit Price Quantity
Sterile Carbohydrate Solution 100 ml 113055034 76019-966 Each (100ml) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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VWR will not lift restrictions for residential shipping addresses.

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SPECIFICATIONS

Storage and Stability Store at Room Temperature (15 - 30 °C)
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