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ISIS DNA Polymerase, MP Biomedicals

Supplier: MP BIOMEDICALS (FKA ICN BIOMED
11EPSIS100 11EPSIS103
For high fidelity PCR.

  • Extremely low error rates
  • Optimal blunt end cloning, Storage: –20 °C
  • The most thermostable polymerase available
  • Storage buffer: Tris HCl 20 mM, pH 8.0, KCl 100 mM, EDTA 0.1 mM, Dithiothreitol 1 mM, Glycerol 50%
  • Incubation: TrisHCl 20 mM, pH (25 °C) 9.0, KCl 25 mM, (NH₄)2SO₄
  • buffer 1X: MgSO₄ 1.5 mM, BSA 0.1 mg/ml Incubation buffer is provided 10X
  • Quality control: Activity, absence of nickases and endonucleases, absence of ribonucleases,specific PCR on genomic and phage
  • templates
  • Unit defi nition: One unit is the amount of enzyme required to catalyze the incorporation of 10 nmol of nucleosides into acid insoluble
  • material in 30 min. at 74 °C under assay conditions

Isis DNA Polymerase does not require primer 3'-end protection, unlike many other proofreading enzymes. This saves time during reaction setup and optimization. Isis DNA polymerase is able to amplify up to 6 kb on genomic DNA templates and 18 kb on phage DNA under a variety of amplification conditions. Isis DNA polymerase offers robust activity and fl exibility. There is a patent pending on the enzyme Isis DNA polymerase. Isis DNA Polymerase has been developed in collaboration with IFREMER.

Application includes: Nucleic Acid Amplification; PCR; High Fidelity PCR; Proofreading PCR.

Delivery information: 100 U Isis™ DNA Pol at 1 U/µl, Isis™ buffer 10XC (1ml) with MgSO4, 1 ml MgSO₄ at 30 mM.



 

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Description Size Supplier No. VWR Catalog Number Unit Price Quantity
Proteins, Enzymes and Peptides 100 U 11EPSIS100 IC11EPSIS100 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

Proteins, Enzymes and Peptides 3×100 U 11EPSIS103 IC11EPSIS103 Pack of 3 Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
  • State issued document with your organization's Resale Tax ID Number
  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

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SPECIFICATIONS

Storage and Handling Store at –20°C.
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