Rosetta™ 2 Competent Cells, Expression Strains, MilliporeSigma

Supplier: MilliporeSigma
71397-3 71402-3 71402-3 71403-3 71397-4 71397-4 71403-4 71403-3 71397-3 71402-4 71403-4 71402-4
82601-964EA 467.65 USD
CA16003-938 82601-964 16003-940 16003-864 16003-942 CA16003-940 CA16003-864 CA16003-942 16003-944 CA16003-944 CA82601-964 16003-938
Rosetta™ 2 Competent Cells, Expression Strains, MilliporeSigma
Competent Cells
Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

  • Supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG)
  • Compatible chloramphenicol-resistant plasmid
  • tRNA genes are driven by their native promoters

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
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