Build highly complex, Illumina-compatible DNA fragment libraries using as little as 50 pg of DNA with these quick and easy library prep and dual indexing kits which are optimized for library construction in 96-well plates.
- Formatted for high throughput: Provides sufficient overfill of each reagent to enable automated or manual multi-channel pipet-based 96-well plate library construction
- Easy-to-use: Two color dual indexing primer sets facilitate visual verification of correct primer dispensing
- High quality results: Same high performance characteristics as the NxSeq® UltraLow DNA library kit, 12 Reactions
- High value: Cost-effective library and indexing kits that produce excellent sequencing results
The NxSeq® UltraLow DNA Library Kit, 96 Reactions and NxSeq® HT Dual Indexing Kit share all the same features and performance characteristics as the smaller, NxSeq UltraLow DNA Library Kit, 12 Reactions and Single Indexing Kits (VWR Cat. No. 76180-800, 76180-802, 76180-804). This 96 reaction kit is formatted for the construction of DNA fragment libraries in 96-well plates and can be used with multichannel pipettes or with automated liquid-handling systems. To facilitate 96-well library prep, we provide sufficient overfill of each reagent and a detailed, step-by-step protocol in the User Manual.
Key features shared between the 96 and 12 reaction kits include the ability to build highly complex DNA fragment libraries from as little as 50 pg of DNA, easy workflows with a minimal number of reagents, improved coverage and better depth uniformity, and low library prep costs with high data quality. Both kits and protocols produce identical, high quality sequencing data. Please visit the NxSeq UltraLow DNA Library Kit, 12 Reactions and Single Indexing Kits VWR catalog webpage for additional performance data.
To aid in accurate dispensing of the NxSeq HT Dual Indexing Primers across a 96-well PCR plate, we added a different inert dye to each set of primers. The 700 Series Indexing Primers contain a blue dye, while the 500 Series Indexing Primers contain an orange/yellow dye. With colored primer sets, you can easily see when each primer is added to the correct row or column of the 96-well PCR plate and verify that all primers are correctly dispensed. We performed extensive testing to verify that these dyes do not interfere with the Illumina instrument or sequencing results.
Both NxSeq UltraLow DNA Library and Indexing Kits were optimized for whole genome sequencing (WGS) and resequencing for de novo sequencing or SNV/mutation or copy number variation (CNV) identification. Both systems, however, are also compatible with other applications such as ChIP-seq, exome-seq, and with other sample types such as FFPE and metagenomic DNA samples.
Ordering information: The NxSeq UltraLow DNA Library Kit, 96 Reactions contains Enzyme Mix (EM), 2X Buffer (2XB), Ligase (LIG), 2X PCR Master Mix (MM) and Elution Buffer (EB). A NxSeq HT Dual Indexing Kit is required to complete library prep and must be purchased separately.
The NxSeq® HT Dual Indexing Kit contains a Universal Adaptor, Adaptor Dilution Buffer and Indexing Primers 701 – 712, and 501 – 508. There is enough of each reagent in this kit to generate 96 different dual indexed libraries. Each NxSeq® HT Dual Index equals the TruSeq® HT Index with the same number.
Caution: For research use only. Not for human or diagnostic use.
