Luciferase Assay Reagent, 100 ml, Promega

Supplier: Promega
E1483
PAE1483EA 897.96 USD
PAE1483
Luciferase Assay Reagent, 100 ml, Promega
Assays Enzyme Assays
An extremely sensitive reagent for rapid quantitation of firefly luciferase. Can be used for assays in single-tube luminometers or in multiwell-plate luminometers with auto-injectors.

  • Ready-to-Use Reagent for Detection of Firefly Luciferase Activity
  • Convenient premixed reagent containing Luciferase Assay Substrate and Buffer, used in the Luciferase Assay System (Cat.# E1500, E1501)
  • Linear over eight or more orders of magnitude of enzyme concentration
  • Sensitive to 10-20 moles of luciferase

The Luciferase Assay Reagent contains Luciferase Assay Substrate and Luciferase Assay Buffer in a convenient premixed format. The reagent is used to detect firefly luciferase activity and is a component of the Luciferase Assay System (Cat. E1500, E1501). The Luciferase Assay Reagent generates light that is nearly constant for at least 1 minute and so is compatible with measuring firefly luciferase in a single-tube luminometer or in a multiwell plate luminometer with an auto-injector. The Luciferase Assay System is a nonhomogeneous assay system; the cells containing the luciferase must be lysed before reagent addition. Glo Lysis Buffer (Cat.# E2661), Cell Culture Lysis Reagent (Cat.# E1531), Passive Lysis Buffer (Cat.# E1941) and Reporter Lysis Buffer (Cat.# E3971) can be used with the Luciferase Assay Reagent in mammalian cells. The Luciferase Assay Reagent also can be used for quantitation in plant and bacterial cells, but only Cell Culture Lysis Reagent is suitable for these applications. Reporter Lysis Buffer enables firefly luciferase, CAT and beta-galactosidase assays to be performed from the same cell extract.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR