Recombinant human ALK4 (150-end) was expressed in Sf9 cells using an N-terminal GST tag. ALK4 is a member of the subfamily of receptor ser/thr kinases that mediates signaling by the Activins.
- Easily Screen and Profile ALK4 Kinase Inhibitors
- Includes kinase, substrate and reaction buffer
- Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
Recombinant human ALK4 (150-end) was expressed in Sf9 cells using an N-terminal GST tag. ALK4 is a member of the subfamily of receptor ser/thr kinases that mediates signaling by the Activins. ALK4 is expressed in many human tissues, including kidney, pancreas, brain, lung and liver. Truncated ALK4, predominantly expressed in human pituitary adenomas, function as dominant negative receptors to interfere with wild-type receptor function and blocks the antiproliferative effect of activin possibly contributing to development of human pituitary tumors. ALK4 is able to mediate Nodal signaling in the presence of Cripto during vertebrate development. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: ALK4, 10microg (Human, recombinant; amino acids 150-end). MW: ~64kDa. Substrate: Native Casein Protein; purified from bovine milk. Other: Reaction Buffer, DTT. ALK4 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/91/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.
