Sepharose™ Protein G is prepared by covalently coupling recombinant Protein G to 6% crosslinked sepharose beads. The coupling technique is optimized to give a high binding capacity for IgG, resulting in IgG binding greater than 10 mg of human IgG per cc of slurry.
Protein G is used for immunoprecipitation and purification of monoclonal and polyclonal antibodies. This product shows stability within pH range of 2 to 10, is high flow rate capable, and has low falling off of rProtein G.
Sepharose™ Protein G binds to all IgG subclasses from human, monkey, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.