ZR Plasmid MiniPrep™ Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol together with a unique fast-spin column to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy determination of complete cell lysis and neutralization. Plasmid DNA purified using this kit is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
- Easy traditional method for isolating high-quality plasmid DNA from E. coli
- Unique column design: zero buffer retention and low (30 µL) elution volume
- Innovative colored P1, P2, and P3 buffers for rapid identification of complete bacterial cell lysis and neutralization steps
This kit yields up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. It is applicable for ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications.
Delivery information: Kit includes Zymo-Spin™ IIN, DNA Elution Buffer (4 mL, 10 mL and 16 mL), Buffer (P1, P2, P3), Plasmid Wash Buffer (24 mL and 48 mL), Endo-Wash Buffer (30 mL), Endo-Wash Buffer (60 mL) and Collection Tubes (2 mL).
