ADP-Glo Kinase Assay + NUAK2 Kinase Enzyme System, 1 each, PromegaSupplier: Promega
PAV5097EA 1146.31 USDPAV5097
ADP-Glo Kinase Assay + NUAK2 Kinase Enzyme System, 1 each, Promega
Assays Enzyme Assays
Recombinant full-length human NUAK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NUAK2 or SNF1/AMP kinase-related kinase (SNARK) is a member of the NUAK family of SNF1-like kinase 2.
- Easily Screen and Profile NUAK2 Kinase Inhibitors
- Includes kinase, substrate and reaction buffer
- Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
Recombinant full-length human NUAK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NUAK2 or SNF1/AMP kinase-related kinase (SNARK) is a member of the NUAK family of SNF1-like kinase 2. NUAK2 is activated by muscle contraction and is a unique mediator of contraction-stimulated glucose transport in skeletal muscle. NUAK2 is involved in cellular stress responses linked to obesity and type 2 diabetes. NUAK2 shows kinase activity against a synthetic test peptide and the activity in keratinocytes is increased by AMP and 5-amino-4-imidazolecarboxamide riboside, implying that AMPK kinase-dependent pathway can activate NAUK2. Glucose deprivation also increases NAUK2 activity in baby hamster kidney fibroblasts. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: NUAK2, 10microg (Human, recombinant full length). MW: ~110kDa. Substrate: CHKtide (KKKVSRSGLYRSPSMPENLNRPR); derived from the human CDC25C protein isoform A (amino acid 205-225). Other: Reaction Buffer, DTT. NUAK2 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/81788/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.
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