ADP-Glo Kinase Assay + MRCK alpha Kinase Enzyme System, 1 each, Promega

Supplier: Promega
V5711
PAV5711EA 1196.72 USD
PAV5711
ADP-Glo Kinase Assay + MRCK alpha Kinase Enzyme System, 1 each, Promega
Assays Enzyme Assays
Recombinant human MRCKalpha (1-473) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

  • Easily Screen and Profile MRCKα Kinase Inhibitors
  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

Recombinant human MRCKalpha (1-473) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Myotonic Dystrophy Kinase-Related Cdc42-Binding Kinase α (MRCKα) is a Cdc42/Rac/Rho interactive/binding serine/threonine kinase with multiple functional domains. MRCKs are effectors of RhoA and Cdc42, respectively, for actin reorganization. MRCKα is a critical regulator of signal transduction pathways in eukaryotic cells that are known principally for their role in regulating the cytoskeleton, and they do so by recruiting a variety of downstream effector proteins. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: MRCKα, 10microg (Human, recombinant; amino acids 1-473). MW: ~73kDa. Substrate: S6K substrate (KRRRLASLR); derived from human 40S ribosomal protein S6 (amino acid 230-238). Other: Reaction Buffer, DTT. MRCKα NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/8476/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.
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