Wizard Plus Megapreps DNA Purification System, 5 preps, Promega®Supplier: Promega
PAA7300EA 329.3 USDPAA7300
Wizard Plus Megapreps DNA Purification System, 5 preps, Promega®
Nucleic Acid Reagents Nucleic Acid Purification Kits and Reagents
A simple, reliable, silica-resin-based method for rapid isolation of plasmid DNA from 1 liter cultures.
- More than 1mg Plasmid from 1L Cultures
- 3-hour protocol
- Resin-based batch column method
- No organic extractions
Wizard Plus Megapreps DNA Purification System provides a simple and rapid method for large-scale purifications of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. The system yields greater than one milligram of high-copy-number plasmid DNA (200-20,000bp) from a 1,000ml culture in less than three hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) or TE buffer and can be used directly for DNA sequencing and restriction digestion without further manipulation. The DNA also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin Ribonuclease Inhibitor (Cat.# N2511).