GoTaq Probe RT-qPCR Systems enable detection and relative quantification of RNA expression levels.
- Simple, One-Step RT-qPCR for RNA Expression Analysis
- Includes GoScript™ Reverse Transcriptase and GoTaq(R) Probe qPCR Master Mix. RNasin(R) RNase protection provided in the supplied GoScript™ RT Mix
- Compatible with fast and standard cycling methods using probe-based assays
- Sensitive detection for quantitation over a broad dynamic range
The GoTaq Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript Reverse Transcriptase and GoTaq Probe qPCR Master Mix in a single-step real-time amplification reaction. The GoScript RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities of GoScript Reverse Transcriptase, RNasin Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination.
Caution: Store all components between –30°C and –10°C. Protect components from light at all times. For best results, mix thawed solutions gently to minimize aeration and foaming, and keep on ice. For short–term storage and frequent use, the GoTaq qPCR Master Mix, 2X, may be kept at 2 to 8°C for up to three months if protected from light.
