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Anti-ERBB2 Mouse Monoclonal Antibody [clone: HRB2/451]

Supplier: Prosci
33-122
This mAb is specific to HER2 / ErbB2 and shows minimal cross-reaction with other members of the family. HER2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. HER2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml IF: 1-2 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 min at Room Temp (1) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the mAb to be titered up or down for optimal performance.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Type: Primary
Antigen: ERBB2
Clonality: Monoclonal
Clone: HRB2/451
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human




 

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Size Supplier No. VWR Catalog Number Unit Price Quantity
100 μg 33-122 76195-506 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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SPECIFICATIONS

Antigen Symbol ERBB2
Antigen Name Erb-B2 Receptor Tyrosine Kinase 2
Reactivity Human
Conjugation Unconjugated
Clonality Monoclonal
Antibody Type Primary
Clone HRB2/451
Host Mouse
Isotype IgG1, kappa
ImmunoChemistry Yes
ImmunoFluorescence Yes
Flow Cytometry Yes
Antigen Synonyms erbB-2, NEU, NGL, HER2, TKR1, CD340, HER-2, MLN 19, HER-2/neu, MLN19, NEU, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein
Gene ID 2064
Concentration 0.2 mg/mL
Format Purified Antibody
Immunogen Recombinant human protein was used as the immunogen for this HER2 antibody.
Form Liquid
Storage Buffer PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Storage Temperature Aliquot and Store at -20C. Avoid freez-thaw cycles.
Shipping Temperature 4°C
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