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Anti-EGF R Mouse Monoclonal Antibody [clone: GFR450]

Supplier: Prosci
<p>This antibody recognizes a protein of 170kDa, identified as EGFR. <a href=../search_result.php?search_txt=egfr>EGFR</a> is type I receptor tyrosine kinase with sequence homology to <a href=../search_result.php?search_txt=erbb2>ErbB2</a>, -3 -4 or <a href=../search_result.php?search_txt=her2>HER2</a>, -3 -4. It binds to Epidermal Growth Factor (EGF), <a href=../search_result.php?search_txt=TGFa>Transforming Growth Factor-a</a> (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head/neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Flow Cytometry: 0.5-1 ug/million cells in 0.1ml IF: 1-2 ug/ml The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Type: Primary
Antigen: EGF R
Clonality: Monoclonal
Clone: GFR450
Conjugation: Unconjugated
Host: Mouse
Isotype: IgG2a, kappa
Reactivity: Human



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Size Supplier No. VWR Catalog Number Unit Price Quantity
100 μg 33-119 76195-500 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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Antigen Symbol EGF R
Antigen Name Epidermal Growth Factor Receptor
Reactivity Human
Conjugation Unconjugated
Clonality Monoclonal
Antibody Type Primary
Clone GFR450
Host Mouse
Isotype IgG2a, kappa
Western Blot Yes
ImmunoChemistry Yes
ImmunoFluorescence Yes
Flow Cytometry Yes
Antigen Synonyms Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Gene ID 1956
Concentration 0.2 mg/mL
Format Purified Antibody
Immunogen The immunizing protein for this antibody was recombinant human EGFR.
Form Liquid
Storage Buffer PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Storage Temperature Aliquot and Store at -20C. Avoid freez-thaw cycles.
Shipping Temperature 4°C
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