Keep my session open?
Ending In 
Your shopping session has expired.
Your session has expired. For your security, we have logged you out.
Would you like to log in again?

Anti-CDKN1B Mouse Monoclonal Antibody [clone: SX53G8]

Supplier: Prosci
33-108
This antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. The p27Kip1 antibody is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. It is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.

Flow Cytometry: 0.5-1 ug/million cells IF: 0.5-1 ug/ml WB: 0.5-1 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the p27Kip1 antibody to be titered up or down for optimal performance.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Type: Primary
Antigen: CDKN1B
Clonality: Monoclonal
Clone: SX53G8
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human;Mouse;Rat;Monkey




 

ORDER

Expand All / Collapse All
Size Supplier No. VWR Catalog Number Unit Price Quantity
100 μg 33-108 76195-478 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
  • State issued document with your organization's Resale Tax ID Number
  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

Generate Barcodes Barcode Label Format: 
Generate PDF Catalog Page

SPECIFICATIONS

Antigen Symbol CDKN1B
Antigen Name Cyclin-Dependent Kinase Inhibitor 1B (P27, Kip1)
Reactivity Monkey, Mouse, Human, Rat
Conjugation Unconjugated
Clonality Monoclonal
Antibody Type Primary
Clone SX53G8
Host Mouse
Isotype IgG1, kappa
Western Blot Yes
ImmunoChemistry Yes
ImmunoFluorescence Yes
Flow Cytometry Yes
Antigen Synonyms Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1
Gene ID 1027
Concentration 0.2 mg/mL
Format Purified Antibody
Immunogen Purified GST-p27 fusion protein of human origin was used as the immunogen for this p27Kip1 antibody.
Form Liquid
Storage Buffer PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Storage Temperature Aliquot and Store at -20C. Avoid freez-thaw cycles.
Shipping Temperature 4°C
Enter Details

Select the article for which you are looking for Certificate

Indicate the Lot Number. This field is mandatory.

Enter Certificate Details

Enter the Lot number:

Choose from recent batch/lot numbers:

Or
Review & Compare Alternatives
We found alternative products that can save you up to  per item-unit.  To compare product details, select up to 3 alternatives below and click Compare Selected. To add items to your basket, enter a quantity and click Add to Basket.

Original Product:

Description Catalog Number Availability Unit Your Price Price Per Qty
 

A new look and a renewed commitment

Built on the features & resources you know, optimized with innovation from Avantor®. Our new brand symbolizes agility and our ability to adapt to customer needs.

Learn More Start Shopping