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Anti-CD63 Mouse Monoclonal Antibody [clone: MX-49.129.5]

Supplier: Prosci
33-100
The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Flow Cytometry: 0.5-1 ug/million cells IF: 0.5-1 ug/ml WB: 1-2 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.<p>1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Type: Primary
Antigen: CD63
Clonality: Monoclonal
Clone: MX-49.129.5
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human;Mouse




 

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Size Supplier No. VWR Catalog Number Unit Price Quantity
100 μg 33-100 76195-462 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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SPECIFICATIONS

Antigen Symbol CD63
Antigen Name Cd63 Molecule
Reactivity Human, Mouse
Conjugation Unconjugated
Clonality Monoclonal
Antibody Type Primary
Clone MX-49.129.5
Host Mouse
Isotype IgG1, kappa
Western Blot Yes
ImmunoChemistry Yes
ImmunoFluorescence Yes
Flow Cytometry Yes
Antigen Synonyms CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63, CD63, MLA1, TSPAN30
Gene ID 967
Concentration 0.2 mg/mL
Format Purified Antibody
Immunogen Full length human CD63 was used as the immunogen for this antibody.
Form Liquid
Storage Buffer PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Storage Temperature Aliquot and Store at -20C. Avoid freez-thaw cycles.
Shipping Temperature 4°C
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