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Anti-CD86 Mouse Monoclonal Antibody [clone: BU63]

Supplier: Prosci
33-093
This antibody recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by Bcell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in Tcell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.

Flow Cytometry: 0.5-1 ug/million cells IF: 1-2 ug/ml IHC (FFPE): 1-2 ug/ml (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD86 antibody to be titered up or down for optimal performance.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Type: Primary
Antigen: CD86
Clonality: Monoclonal
Clone: BU63
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human;Mouse;Rat




 

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Size Supplier No. VWR Catalog Number Unit Price Quantity
100 μg 33-093 76195-448 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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SPECIFICATIONS

Antigen Symbol CD86
Antigen Name Cd86 Molecule
Reactivity Rat, Human, Mouse
Conjugation Unconjugated
Clonality Monoclonal
Antibody Type Primary
Clone BU63
Host Mouse
Isotype IgG1, kappa
ImmunoChemistry Yes
ImmunoFluorescence Yes
Flow Cytometry Yes
Antigen Synonyms T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B72, FUN-1, CD86, CD86, CD28LG2
Gene ID 942
Concentration 0.2 mg/mL
Format Purified Antibody
Immunogen ARH-77 (B-lymphoblastoid cell line) was used as the immunogen for this CD86 antibody.
Form Liquid
Storage Buffer PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Storage Temperature Aliquot and Store at -20C. Avoid freez-thaw cycles.
Shipping Temperature 4°C
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