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Anti-CD8A Rabbit Polyclonal Antibody

Supplier: Prosci
33-088
CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha-beta) or as a homodimer (CD8 alpha-alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4. CD8 alpha antibody is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.

Flow Cytometry: 1-2 ug/million cells IF: 1-2 ug/ml The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Type: Primary
Antigen: CD8A
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human




 

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Size Supplier No. VWR Catalog Number Unit Price Quantity
100 μg 33-088 76193-838 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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SPECIFICATIONS

Antigen Symbol CD8A
Antigen Name Cd8A Molecule
Reactivity Human
Conjugation Unconjugated
Clonality Polyclonal
Antibody Type Primary
Host Rabbit
Isotype IgG
ImmunoFluorescence Yes
Flow Cytometry Yes
Antigen Synonyms T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL
Gene ID 925
Concentration 0.2 mg/mL
Format Purified Antibody
Immunogen Human CD8 recombinant fragment corresponding to aa 200-250 was used as the immunogen for this antibody.
Form Liquid
Storage Buffer PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Storage Temperature Aliquot and Store at -20C. Avoid freez-thaw cycles.
Shipping Temperature 4°C
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