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Anti-CA9 Mouse Monoclonal Antibody [clone: 66.4.C2]

Supplier: Prosci
33-073
This antibody recognizes a glycoprotein of ~200kDa, identified as renal cell carcinoma protein, carbonic anhydrase IX / CAIX, or gp200. Its epitope resides in the carbohydrate domain of the protein. The CAIX antibody shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, CAIX is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, it is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This CAIX antibody may be useful in the investigations of carcinomas of proximal nephrogenic differentiation, especially those showing tubular differentiation.

Flow Cytometry: 0.5-1 ug/million cells IF: 1-2 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CAIX antibody to be titered up or down for optimal performance.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Type: Primary
Antigen: CA9
Clonality: Monoclonal
Clone: 66.4.C2
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b, kappa
Reactivity: Human;Horse




 

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Size Supplier No. VWR Catalog Number Unit Price Quantity
100 μg 33-073 76195-432 Each (100µG) Retrieving Restricted

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SPECIFICATIONS

Antigen Symbol CA9
Antigen Name Carbonic Anhydrase Ix
Reactivity Horse, Human
Conjugation Unconjugated
Clonality Monoclonal
Antibody Type Primary
Clone 66.4.C2
Host Mouse
Isotype IgG2b, kappa
ImmunoChemistry Yes
ImmunoFluorescence Yes
Flow Cytometry Yes
Antigen Synonyms Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, CA-IX, CAIX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, RCC-associated antigen G250, pMW1, CA9, G250, MN
Gene ID 768
Concentration 0.2 mg/mL
Format Purified Antibody
Immunogen The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for this Carbonic Anhydrase IX / CAIX antibody.
Form Liquid
Storage Buffer PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Storage Temperature Aliquot and Store at -20C. Avoid freez-thaw cycles.
Shipping Temperature 4°C
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