Trypan Blue 0.4% in PBS, dark blue solution


Synonyms: CI 23850Sodium 3,3'-((3,3'-dimethyl-[1,1'-biphenyl]-4,4'-diyl)bis(diazene-2,1-diyl))bis(5-amino-4-hydroxynaphthalene-2,7-disulfonate)Direct Blue 14AzidinBlueTolidine-disazo-bis-8-amino-1-naphthol-3,6-disulfonic acid tetrasodium saltBenzamine blueTrypan blue sodium salt3,3′-Dimethyl-4,4′-bis(5-amino-4-hydroxy-2,7-disulfonaphthyl-3-azo)-[1,1′-biphenyl] tetrasodium salt

76180-676EA 27.11 USD
Trypan Blue 0.4% in PBS, dark blue solution
Trypan Blue

Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxicity and proliferation assays.
Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis and Sauter's fluorescence method and for the staining of collagen, including very fine fibrils, muscle and cornified epithelium using the Van Gieson method. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.

  • Suitable for cell culture
  • Presentation: Dark Blue Solution
  • Potassium Phosphate Dibasic: 600.00000 mg/L, Sodium Chloride [NaCl]: 8100.00000 mg/L, Trypan Blue: 4000.00000 mg/L
  • Soluble in ethylene glycol monomethyl ether (methyl cellosolve; EGME) (20 mg/mL); Slightly soluble in water or ethanol (0.6 mg/mL); almost insoluble in alcohol.

Formula: C₃₄H₂₄N₆Na₄O₁₄S₄
MW: 960.82 g/mol
MDL Number: MFCD00003969
CAS Number: 72-57-1

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Specification Test Results

pH 7.0-8.0
Osmolality 270-340mOsm
Cell Dye Uptake ≥90%
Dye Concentration ((A594± 12nm) ≤0.5%

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