Easy purification of high quality genomic DNA from whole blood or buffy coat samples
The MasterPure™ DNA Purification Kit for Blood Version II can be used to isolate exceptionally pure genomic DNA from whole blood or buffy coat. With the optimized protocol for DNA, up to 100 µg of DNA can be recovered from 600 µl of buffy coat in less than 30 minutes. The purified DNA contains both high- and low-molecular-weight genomic DNA which can be used directly as a template in PCR amplification reactions, for digestion with restriction enzymes, for Southern blotting, or for other molecular biology applications. Protocols are provided for genomic DNA and total nucleic acid purification from whole blood and buffy coat. For RNA samples, we recommend the MasterPure RNA Purification Kits. Both protocols allow for scaling up to accommodate larger samples.
Scalable purification of gDNA from blood
Consistent yields of up to 10 µg of DNA from 325 µL of whole blood and approximately 100 µg DNA from 600 µL of buffy coat
High purity gDNA with A260/A280 ratios of 1.8-2.0 making the DNA suitable for most downstream molecular biology applications including PCR and sample archiving
Fast, 30 minute protocol
Better safety since no hazardous organic solvents are used
For DNA purification from a total of 400 mL blood
