R&DNA™ T7 Polymerase, 'RNA Synthesis' with NTPs and dNTPs, Biosearch Technologies

Supplier: Biosearch Technologies
D7P9205K
76081-618EA 1288.62 USD
76081-618
R&DNA™ T7 Polymerase, 'RNA Synthesis' with NTPs and dNTPs, Biosearch Technologies
Nucleic Acid Reagents RNA Synthesis and Transcription
Synthesize "RNA" transcripts that also contain both standard or modified dNTPs using this mutant T7 RNA polymerase

  • Generate "RNA" transcripts with both rNMP/2´-dNMP or rNMP/2´-modified-NMP composition
  • Create modified "RNA" transcripts that are resistant to RNase A-type Rnases
  • Use for a variety of applications where "RNA" with incorporated dNTPs is beneficial

T7 R&DNA™ Polymerase* is a recombinant mutant form of T7 RNA polymerase (Y639F mutant), in which Tyr-639 has been replaced by Phe. This mutation enables T7 R&DNA Polymerase to incorporate 2´-deoxyribonucleoside triphosphates (dNTPs) into full-length transcripts much more efficiently than the corresponding wild-type enzyme, while retaining the same catalytic activity for incorporation of canonical NTPs, and the same high promoter specificity as wild-type T7 RNA Polymerase

Note: Complete substitution of one 2´-dNTP (or one 2´-modified dNTP) for a canonical rNTP in a T7 R&DNA Polymerase reaction results in a slight decrease in yield. Additional substitutions of 2´-dNTPs (or 2´-modified-dNTPs) for canonical rNTPs will further reduce yield of the transcript produced. Substitution with all four 2´-dNTPs (or 2´-modified dNTPs) will result in extremely low yields of transcript and is NOT RECOMMENDED.

The ability of this mutant enzyme to incorporate certain 2´-modified-dNTPs (including but not limited to dNTPs having a 2´-fluorine or 2´-amino group) enables efficient in vitro synthesis of transcripts composed of mixed rNMP/2´-dNMP or rNMP/2´- modified-dNMP from any DNA template that is downstream of the T7 RNA polymerase promoter. Such modified transcripts are useful for many applications.

Certifications: For research use only. Not for human or diagnostic use.

Ordering information: Contents include T7 R&DNA™ Polymerase, 5X Reaction Buffer, and 100 mM DTT.
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