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Pierce EZ-Link® Solid Phase Biotinylation Kits, Thermo Scientific

Supplier: Thermo Scientific
EZ-Link®
21930 21440 21450
This antibody labeling system uses nickel chelated agarose to temporarily immobilize antibody molecules via their histidine-rich Fc regions. Once held in place on the gel, the antibody can be biotinylated at either sulfhydryl groups (after mild reduction or disulfide bonds) or primary amines. Excess labeling reagent and by-products are then washed away before recovering the labeled and purified antibody from the gel using a mild imidazole solution. No gel filtration or dialysis are needed.

  • Fast labeling and purification, the entire procedure takes only one hour (two hours for sulfhydryl labeling kits)
  • Easy removal of spent and excess labeling reagent, no need for dialysis or gel filtration
  • Solid phase method allows initially dilute antibodies to be recovered in a smaller volume after labeling
  • Specific protocols for antibody ensure appropriate level of labeling (2 to 5 biotins per antibody molecule), minimising possibility of inactivation caused by overlabeling
  • No-Weigh™ single-dose microtube packaging ensures that the biotin reagent is fully active for eight separate experiments

A single sealed microtube containing 2 mg of reagent is reconstituted for each biotinylation. The No-Weigh™ packaging allows access to fresh reagent on-demand for each solid phase biotinylation reaction.




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Description No. of Reactions Supplier No. VWR Catalog Number Unit Price Quantity
Solid Phase Biotinylation NHS-PEO Pre-Packed Column Kit 1x1 mL column biotinylates 1 - 10 mg of antibody (can be reused 10 times) 21440 PI21440 Each Retrieving

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Solid Phase Biotinylation Maleimide-PEO Mini-Spin Column Kit 1x1 mL column biotinylates 1 - 10 mg of antibody (can be reused 10 times) 21930 PI21930 Each Retrieving

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Solid Phase Biotinylation NHS-PEO Mini-Spin Column Kit 10 discs, each disc can biotinylate 100 - 1000 μg of antibody 21450 PI21450 Each Retrieving

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

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