Easy-Titer® IgM Assay Kit is a simple, mix-and-read assay that allows quick and accurate determination of concentrations from 15 to 500 ng/mL of IgM
- Faster and easier than ELISA
- Sensitive, requires small sample volume
- No special equipment needed
- Highly reproducible results
The assay procedure uses monodispersed polystyrene beads that are coated with anti-human IgM antibodies and absorb light at 340 and 405 nm. When the beads are mixed with a sample containing human IgM, they aggregate, causing decreased absorption of light and, therefore, low IgM concentrations yield high absorbance values and high IgM concentrations yield low absorbance values. The decrease in absorption is proportional to IgM concentration and a standard curve can be generated to accurately quantify IgM levels in serum or citrated plasma samples.
Delivery information: Kit include sufficient components for 96 tests. An IgM standard must be purchased separately. Each kit contains goat Anti-Human IgM Coated polystyrene beads (2 mL), Dilution buffer (30 mL), and blocking reagent (15 mL).