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Anti-pRB Mouse Monoclonal Antibody [clone: 2C4]

Cyclin-dependent kinases (Cdks) play important roles in the regulation of the cell cycle. RB protein (pRB) is phosphorylated by cyclin D-Cdk4/Cdk6 and cyclin A/cyclin E-Cdk2 during the G1/S transition. This phosphorylation causes the inactivation of the growth inhibitory functions of pRB. pRB undergo phosphorylation and attendant functional inactivation, the cell proceed into late G1. Cyclin D-Cdk4 specifically phosphorylates Ser780 in pRB, while cyclin E-Cdk2 and cyclin A-Cdk2 does not.

WB: 1 ug/ml

Type: Primary
Antigen: pRB
Clonality: Monoclonal
Clone: 2C4
Conjugation: Unconjugated
Host: Mouse
Isotype: IgG1
Reactivity: Human



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Size Supplier No. VWR Catalog Number Unit Price Quantity
100 µg 10R-P122A 101983-784 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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Antigen Symbol pRB
Reactivity Human
Conjugation Unconjugated
Clonality Monoclonal
Antibody Type Primary
Clone 2C4
Host Mouse
Isotype IgG1
Western Blot Yes
Modification Phosphoa-SER780
Format Supplied as a lyophilized powder. Reconstitute in 100 ul of distilled water.
Immunogen Phospho-Rb (SER780) antibody was raised in mouse using synthetic human phosoho-RB(ser780) peptide, TRPPTLS(PO3)PIPHIP, which corresponds to amino acids 774-786 of human pRB as the immunogen.
Storage Temperature Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze-thaw cycles.
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