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Anti-PRAS40 Rabbit Polyclonal Antibody

PRAS40 (proline-rich Akt substrate of 40 kDa) is a protein with a proline-rich domain and appears at about 40 kDa by SDS PAGE. Initially it was identified as a substrate of PKB/Akt, it was later shown that it is also phosphorylated by mTORC1 (a complex including scaffold protein which is necessary for mTOR and phosphorylation of substrate by mTOR). PRAS40 binds to raptor through the mediation of the TOS motif in its structure in the same way as p70 S6 kinase and 4EP1 do as substrates of mTOR, then PRAS40 is phosphorylated by mTORC1 at Ser183 (human), and the phosphorylated PRAS40 will be dissociated from raptor. Therefore, it is considered that PRAS40 is involved in signal transducing pathway through the mediation of not only PKB/Akt but also mTOR.

WB: 13 ug/ml

Type: Primary
Antigen: PRAS40
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Rabbit
Isotype: IgG
Reactivity: Human



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Size Supplier No. VWR Catalog Number Unit Price Quantity
100 µg 70R-7768 102030-504 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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Antigen Symbol PRAS40
Reactivity Human
Conjugation Unconjugated
Clonality Polyclonal
Antibody Type Primary
Host Rabbit
Isotype IgG
Western Blot Yes
Format Lyophilized product from 1% BSA in PBS containing 0.05% NaN3, reconstitute with 1 ml of deionised water
Immunogen PRAS40 (S183 phosphorylated) antibody was raised in rabbit using a synthetic peptide of the phosphorylated part of human PRAS40 (QYAK(pS)LPVS) as the immunogen
Storage Temperature Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at 20 deg C for long term storage
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