Ampicillin trihydrate
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
Ampicillin is a semi-synthetic derivative of penicillin, active as a broad-spectrum antibiotic.
- At 25 °C ,pKa = 2.5 (-COOH),pKa = 7.3 (-NH2) (Lit.)
- Physical form: Powder
- Concentration: 100 mg/liter for both gram positive and gram negative bacteria. It is typically stable in media at 37 °C for approximately 3 days.
It is inactivated by beta-lactamases and for this reason a beta-lactamase inhibitor should be considered when using ampicillin
A β-lactam antibiotic with an amino group side chain attached to the penicillin structure. Penicillin derivative that inhibits bacterial cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidases on the inner surface of the bacterial cell membrane. Bactericidal only to growing Escherichia coli. Mode of resistance: Cleavage of β-lactam ring of ampicillin by β-lactamase. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.
Ampicillin Trihydrate is commonly used to select for ampicillin resistance in mutated and transformed cells. It is also described for the use of inhancing luminol chemiluminescence.
Against gram-positive bacteria, ampicillin has a similar mode of action as benzylpenicillin; against gram-negative bacteria, it has a similar mode of action as chloramphenicol and tetracyclines. In E. coli it inhibits cell wall synthesis.
Soluble in 1 M Ammonium Hydroxide (50 mg/mL), dilute acids (50 mg/mL 1 M HCl) or bases; slightly soluble in water (10 mg/mL); practically insoluble in alcohol, chloroform, ether and fixed oils.
Caution: Autoclaving solutions of ampicillin will destroy activity. Solutions can be sterilized by filtration (0.22 um filter) and stored frozen (-20 °C or below). Frozen solutions can be stored for up to 3 months.
Formula:
C₁₆H₁₉N₃O₄S·3H₂O MW: 403.45 g/mol Melting Pt: 198…200 °C (lit.) Storage Temperature: Refrigerator |
MDL Number:
MFCD00072036 CAS Number: 7177-48-2 |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...