RepliQa HiFi Assembly Mix, Quantabio

95190-050
76046-856KT 596.64 USD
76046-856
RepliQa HiFi Assembly Mix, Quantabio
Quantabio’s RepliQa HiFi Assembly Mix is a one-step, isothermal reaction for the cloning of single and multi-insert assemblies eliminating the need of restriction enzymes or restriction sites while outperforming competitor assembly mixes with improved cloning efficiencies and lower overall total costs.

  • Formulated to increase number of transformants
  • Assemble up to 6 fragment inserts without the need for restriction enzymes
  • Flexibility in design with 10X MM enabling assembly of low concentration DNA samples
  • Eliminates dilution step resulting in easier workflow and 1 hr assembly
  • Includes DpnI to reduce background when using plasmid templates for PCR

The RepliQa™ HiFi Assembly Mix simplifies the construction of recombinant DNA through the simultaneous and scarless assembly of multiple DNA fragments possessing terminal regions of sequence overlap in a single, isothermal reaction

Double stranded DNA fragments for assembly can be generated by PCR amplification, chemical synthesis, or isolation of restriction fragments. When working with fragments PCR amplified from plasmid vectors, the included DpnI restriction endonuclease can be used for selectively digesting methylated, residual plasmid DNA to reduce background transformants. The RepliQa mix is directly compatible with most common E. coli cloning hosts and generally provides a high yield of accurately assembled product.

Similar in principle to the Gibson Assembly® Method, the high efficiency RepliQa HiFi Assembly Mix is ideal for a range of genetic engineering applications including routine molecular cloning, site-directed mutagenesis, assembly of large constructs for synthetic biology applications, and the construction of diverse sequence libraries for directed evolution studies. The concentrated, two-component format allows flexibility in design of assembly reactions and compatibility with less concentrated DNA samples. The RepliQa Mix has been optimized for use with a total input quantity of DNA fragments in the range of 0.03 to 0.5 pmols. The assembly of up to six DNA fragments is recommended, though the RepliQa Mix has been successfully used for more complex assemblies.
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