EZ-Tn5™ <KAN-2>Tnp Transposome™ Kit , Transposon Mutagenesis, Tn5 Transposome

TSM99K2
75928-004EA 1035.86 USD
75928-004
EZ-Tn5™ <KAN-2>Tnp Transposome™ Kit , Transposon Mutagenesis, Tn5 Transposome
EZ-Tn5™ <KAN-2>Tnp Transposome™ Kit

Generate random gene knockouts in non-E. coli bacteria for metagenomics and bacterial strain engineering.


  • Generate mutants with improved genetics or function
  • Characterize novel genes and gene functions
  • Identify essential genes involved in pathogenesis, toxicity, biofilm development
  • Unravel metabolic pathways
  • Identify essential genes and regulatory elements
  • 100’s of citations for many different applications


Create and sequence bacterial mutants faster with in vivo transposomics tools


Transposomes are used for in vivo mutagenesis for a broad range of bacteria, including Gram positive and Gram negative strains. The transposome is a complex of an engineered hyperactive Tn5 Transposase enzyme, and a DNA sequence (transposon) to be inserted. The transposon sequence in the Z-Tn5™ <KAN-2>Tnp Transposome™ Kit consists of a broad host-range kanamycin resistance marker, which can be used for insertional mutagenesis and downstream sequencing of the transposon insertion site. Transposomics is easier than chemical mutagenesis, and is a trusted and well-established method for generating mutations across over 60 species of bacteria.


To generate a library of mutants from your organism, simply transform your bacterial strain of interest with the transposome, and grow colonies on selective (kanamycin) media. The colonies can be expanded and screened in almost any way you can imagine - with a phenotypic screen designed to identify a particular attribute, a genetic screen for a specific gene disruption event, or a functional screen (for loss of gene function). After "hits" are identified, the clone can be isolated and further characterized. The transposition insertion site can be sequenced.


EZ-Tn5 Transposome-mediated insertions have been made in many different microorganisms, incluing Acinetobactor, Campylobacter, Escherichia, Mycobacterium, Proteus, Pseudomonas, Saccharomyces, Salmonella, Trypanosoma, Xylella, and others. The number of transposition clones obtained is highly dependent on the transformation efficiency of the host cell.


Caution: For research use only. Not for human or diagnostic use.

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