Insert a T7 promoter to drive gene expression for functional analysis.
- Insert T7 promoter randomly into any DNA sequence in vitro
- Express resulting products in vivo or in vitro
- Minimize insertion bias with the hyperactive modified Tn5 system, known for lowest insertion bias
Identify functional characteristics of novel genes, create and study truncation products with in vitro transposon tools
The EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit* provides an easy and reliable method to randomly insert a transposon containing a phage T7 RNA polymerase promoter into any DNA molecule in vitro. The transposon end has no termination sequences, so RNA can be produced from chosen insertion clones by in vitro transcription from the T7 RNA polymerase promoter using, for example, any of Epicentre's T7 RNA Polymerase Transcription Kits. RNA can also be generated for in vivo expression studies in cells having an inducible T7 RNA polymerase gene.
*Covered by issued and/or pending patents, exclusively licensed or assigned to Epicentre® (an Illumina® Company).
Randomly insert T7 promoter and kanamycin resistance marker (the provided transposon sequence) into purified plasmid or genomic DNA to generate a library of expression clones for in vivo (E. coli) expression, or in vitro transcription and translation studies.
Delivery information: Each kit contains EZ-Tn5™ Transposase, EZ-Tn5™ <T7/KAN-2> Transposon, EZ-Tn5™ 10X Reaction Buffer, EZ-Tn5™ 10X Stop Solution, Forward and Reverse Primers, Control Target DNA, Sterile Wate
Caution: For research use only. Not for human or diagnostic use.