Produce amplified cDNA from precious, limiting cell samples without introducing bias.
- Direct cDNA Amplification: Perform RNA amplification directly from cells followed cDNA synthesis
- No Bias Introduced: High fidelity, linear RNA amplification process that preserves the relative transcript abundance of the sample
- Sensitive: Readily and reproducibly detect even low-abundance transcripts from as few as one cell
- Extend Precious Samples: Produce enough cDNA for multiple qPCR reactions, archiving and future use
- Simple: No RNA purification required
The MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit enables the user to perform sensitive qRT-PCR studies from very small populations of cells, even from a single cell.
The kit amplifies the cellular mRNA directly from a cell lysate, without the need to purify total RNA, and then converts the amplified RNA to cDNA that is ready for qPCR. Amplified cDNA is efficiently produced from the lysates of 1 to 500 cells.
Delivery information: Each kit contains QuickExtract™ RNA Extraction Solution, MessageBOOSTER™ T7-Oligo(dT) Primer, MessageBOOSTER™ Reverse Transcription PreMix, RNase Inhibitor, MMLV Reverse Transcriptase, MessageBOOSTER™ DNA Polymerase PreMix, MessageBOOSTER™ DNA Polymerase, MessageBOOSTER™ cDNA Finishing Solution, MessageBOOSTER™ In Vitro Transcription PreMix, MessageBOOSTER™ T7 RNA Polymerase, MessageBOOSTER™ T7 Transcription Buffer, RNase-Free DNase I, Random Primers, RNase H, Forward Control PCR Primer, Reverse Control PCR Primer, 100 mM DTT, RNase-Free Water, Poly(I).
Caution: For research use only. Not for human or diagnostic use.
