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MycoAlert™ Mycoplasma Detection Kits, Lonza

Supplier: LONZA WALKERSVILLE INC
LT07-710 LT07-218 LT07-701 LT07-118 LT07-418 LT07-318 LT07-703 LT07-518 LT07-705
The MycoAlert™ PLUS Mycoplasma Detection Kit is the next generation MycoAlert™ Kit. It provides higher light output to allow for use with less sensitive plate luminometers or multifunctional readers and testing of unused media, media supplements or water.

  • Convenient & fast
  • Simply add two reagents to the culture supernatant, perform two luminescence readings and get your results in less than 20 minutes
  • Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma)
  • Suited for tube luminometers, plate luminometers and multifunctional readers

The MycoAlert™ PLUS Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells

MycoAlert™ Assay Control Set (available separately)
The MycoAlert™ Assay Control Set includes a lyophilized positive control and assay buffer for reconstitution. The assay buffer also serves as a negative control. The positive and negative controls are designed for use with the MycoAlert™ PLUS Mycoplasma Detection Kit. It is recommended to always include the Control Set to ensure that the operator and all of the assay reagents are performing properly. The positive control is proprietary, but does NOT contain mycoplasma. The positive or negative control is simply substituted for a sample in the standard assay protocol. The positive control will give a ratio of >1 while the negative control will give a ratio of <1.

Viable mycoplasma in a test sample (cell supernatant or fresh media or supplements) are lysed and the enzymes react with the MycoAlert™ PLUS Substrate, catalyzing the conversion of ADP to ATP. The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlert™ PLUS Reagent. By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert™ PLUS Substrate (read B), a ratio can be obtained which is indicative of the presence or absence of mycoplasma.




 

ORDER

Expand All / Collapse All
Description Size Supplier No. VWR Catalog Number Unit Price Quantity
MycoAlert™ Plus Mycoplasma Detection Kits 10 Tests LT07-701 75860-358 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

MycoAlert™ Plus Mycoplasma Detection Kits 30 Tests LT07-703 75860-360 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

MycoAlert™ Plus Mycoplasma Detection Kits 50 Tests LT07-705 75860-362 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

MycoAlert™ Plus Mycoplasma Detection Kits 100 Tests LT07-710 75866-212 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

MycoAlert™ Mycoplasma Detection Kits 10 Tests LT07-118 75870-452 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

MycoAlert™ Mycoplasma Detection Kits 25 Tests LT07-218 75870-454 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

MycoAlert™ Mycoplasma Detection Kits 100 Tests LT07-318 75870-456 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

MycoAlert™ Mycoplasma Detection Kits 50 Tests LT07-418 75870-458 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

MycoAlert™ Assay Control Set 10 Tests LT07-518 75870-450 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
  • State issued document with your organization's Resale Tax ID Number
  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

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