DRG® TM-CA 125 ELISA, DRG InternationalSupplier: DRG International
75871-356KT 227.9 USD75871-356
DRG® TM-CA 125 ELISA, DRG International
An enzyme immunoassay for the quantitative measurement of CA 125 in serum and plasma.
- High quality assays with reproducible and reliable results
- Ready-to-use reagents with internal controls
- Very good precision and sensitivity
- Short assay time and incubation steps at room temperature
- Simple and technician friendly tests
The TM CA 125 ELISA is an assay for the detection of OC 125 reactive determinants on a heterogeneous, high-molecular-weight (200 - 1,000 kDa) glycoprotein in serum
The DRG TM‑CA 125 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site of the CA 125 molecule. An aliquot of patient sample containing endogenous CA 125 is incubated in the coated well with enzyme conjugate, which is a monoclonal anti-CA 125 antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is proportional to the concentration of CA 125 in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CA 125 in the patient sample.
This glycoprotein was originally defined by the OC 125 monoclonal antibody established by Bast et al. OC 125 reactive determinants can be found in a high percentage of non-mucinous epithelial ovarian tumors and are found in the serum of women bearing such tumors. CA 125 values are increased in most patients with active epithelial ovarian