DRG® TM-CYFRA 21-1 ELISA, DRG International

Supplier: DRG International
75871-334KT 402.54 USD
DRG® TM-CYFRA 21-1 ELISA, DRG International
Assays ELISAs
An enzyme immunoassay for the quantitative measurement of CYFRA21‑1 in serum and plasma.

  • High quality assays with reproducible and reliable results
  • Ready-to-use reagents with internal controls
  • Very good precision and sensitivity
  • Short assay time and incubation steps at room temperature
  • Simple and technician friendly tests

Cytokeratins are epithelial markers whose expression is not lost during malignant transformation. CYFRA 21‑1 is a cytokeratin-19 fragment that is soluble in serum and can be used as circulating tumor marker. Although expressed in all body tissues, its major occurrence is in the lung, particulary in lung cancer tissues. CYFRA 21-1 is a sensitive and specific tumor marker of non-small-cell lung cancer (NSCLC), especially of squamous cell subtype. It also reflects the extent of the disease and has an independent prognostic role along with performance status and disease stage in NSCLC. In addition, detection of serum CYFRA 21-1 allows for identification of high risk patients that may benefit from adjuvant chemotherapy, and enables the early detection of progressive disease in recurrent NSCLC. Additionally, CYFRA 21-1 has been described as a useful marker for esophageal squamous cell carcinoma and for therapy monitoring of bladder cancer. The TM‑CYFRA 21‑1 ELISA uses the two mouse monoclonal antibodies KS19.1 and BM19.21 to determine cytokeratin-19 fragments.

The DRG TM‑CYFRA 21‑1 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site of the CYFRA21‑1 molecule. An aliquot of patient sample containing endogenous CYFRA21‑1 is incubated in the coated well with enzyme conjugate, which is an anti- CYFRA21‑1 monoclonal antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of CYFRA21‑1 in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CYFRA21‑1 in the patient sample.
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