DRG® Ureaplasma Urealyticum IgM ELISA, DRG InternationalSupplier: DRG International
75871-368KT 199.23 USD75871-368
DRG® Ureaplasma Urealyticum IgM ELISA, DRG International
An enzyme immunoassay for the qualitative and semiquantitative determination of IgM-class antibodies to ureaplasma urealyticum in serum and plasma.
- High quality assays with reproducible and reliable results
- Ready-to-use reagents with internal controls
- Very good precision and sensitivity
- Short assay time and incubation steps at room temperature
- Simple and technician friendly tests
Ureaplasma urealyticum is a bacterium belonging to the family Mycoplasmataceae. This microorganism is part of the normal genital flora of both men and women. It is found in about 70% of sexually active humans. It can cause disease, including non-specific urethritis(NSU), infertility, chorioamnioitis, stillbirth, premature birth, and, in the perinatal period, pneumoniaormeningitis. The DRG Ureaplasma urealyticum IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Patient samples are diluted with Sample Diluentand additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results. Microtiter wells as a solid phase are coated with Ureaplasma urealyticum antigen. Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Ureaplasma urealyticum-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti‑IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Ureaplasma urealyticum-specific IgM antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.