QuantiLum Recombinant Luciferase is a luciferase expressed from a cloned gene from the North American firefly (Photinus pyralis).
- Reliable and Dependable Recombinant Luciferase
- Available in bulk for large orders
- Custom options available
- Supplied at a concentration of 10-15mg/ml
- Source: an E. coli strain that carries a plasmid for the cloned luciferase gene from the North American firefly Photinus pyralis
QuantiLum Recombinant Luciferase is a luciferase expressed from a cloned gene from the North American firefly (Photinus pyralis) that provides the reliability and dependability needed for performing research or producing kits using bioluminescence reagents to detect ATP or luciferin substrates. A recombinant source eliminates the possibility of seasonal and regional variability that may be found in luciferase purified from natural sources.
The light unit value is the light output generated at room temperature over a 10-second period. QuantiLum Recombinant Luciferase is diluted 106 fold in 1X Cell Culture Lysis Reagent and 1 mg/mL acetylated BSA. A quantity of 10 μL of diluted luciferase is combined with 100 μL of Luciferase Assay Reagent prepared from Luciferase Assay Substrate and Luciferase Assay Buffer.
