GoTaq MDx Hot Start Polymerase contains GoTaq MDx DNA Polymerase bound to a proprietary antibody that blocks polymerase activity.
- Inhibits Polymerase Activity at Temperatures below 70°C
- Manufactured under cGMP
- High-concentration and glycerol-free formulations also available
- All formulations supplied at ≥5u/microl, except for high-concentration formulation, which is supplied at ≥50u/microl
- Custom formats and formulations
GoTaq MDx Hot Start Polymerase contains GoTaq MDx DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94-95°C for two minutes. This allows hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Hot-start PCR is advantageous for some amplification targets because primer-dimer and secondary products are eliminated or minimized. In some cases, hot-start PCR may improve yields. The glycerol-free product formulation is further purified to remove glycerol, making it suitable for further manufacture processing and lyophilization. GoTaq MDx DNA Polymerase products are manufactured under cGMP. GoTaq MDx DNA Polymerase products are general purpose reagents intended for general laboratory use. They can be used as a component of molecular diagnostic assays, where applicable country laws allow, without paying royalties. The products by themselves do not provide any diagnostic result.
The glycerol-free product formulation is further purified to remove glycerol, making it suitable for further manufacture processing and lyophilization. GoTaq MDx DNA Polymerase products are manufactured under cGMP standards.
The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94 to 95°C for two minutes. This allows hot-start PCR, in which polymerase activity is inhibited at temperatures below 70°C. Hot-start PCR is advantageous for some amplification targets because primer-dimer and secondary products are eliminated or minimized. In some cases, hot-start PCR may improve yields.
