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P450-Glo™ CYP450 Assay Systems, Promega

Supplier: Promega Corporation
The P450-Glo CYP450 Assays provide a homogeneous, luminescent method for measuring cytochrome P450 activity.

  • Broad Dynamic Range, Low Background, and Better Sensitivity for Less Ambiguous Data
  • Luminescent Output Eliminates Interference From Fluorescent Test Compounds
  • Homogeneous Assay with Simple Add-and-Read Format
  • Special Formulation Results in Low False-Hit Rate
  • Scalable to 384-Well Format, Reducing Cost per Well

They're designed to measure the activities of P450s from recombinant and native sources and for testing the effects of analytes on P450 activities. These luminescent assays exhibit high sensitivity, low background signals, and broad dynamic range. They employ luminogenic P450 substrates that are derivatives of beetle luciferin. The derivatives aren't substrates for luciferase but are converted by P450s to luciferin, which in turn reacts with luciferase to produce light that is proportional to the activity of the P450. The P450-Glo Assays generate a glow-type luminescent signal, produced using derivatized luciferins as P450 substrates and a recombinant stabilized luciferase (Ultra-Glo™ Luciferase) coupled with a proprietary buffer system. The half-life of the luminescent output is more than two hours, eliminating the need for luminometers with injectors and allowing batch plate processing. The formulation also reduces the incidence of false positives due to inhibition of luciferase by analytes when screening for cytochrome P450 inhibitors.

Drug-induced changes in expression of CYP450 genes are a key cause of drug interactions. The ability to measure enzymatic activity of induced specific human isoforms is critical for developing safer drugs. The new P450-Glo CYP2B6 Assay measures the activities of the most important inducible human isoforms: CYP1A2, CYP2B6, and CYP3A4. The luciferin-based substrates are taken up by cells and rapidly converted into luciferin inside the cells, which reduces the incubation time required (typically 30–60 minutes). The low background and high signal-to-noise ratios mean less starting material is required. Dimethyl sulfoxide (DMSO), a common solvent used to solubilize chemical compounds, can significantly inhibit the activity of the 3A4 isoform of cytochrome P450 even at low concentrations. The P450-Glo CYP3A4 System (Luciferin-PPXE) DMSO-Tolerant Assay tolerates DMSO in the 3A4 reaction. The assay exhibits little-to-no change in the signal-to-background ratio in the presence of 0.2% DMSO compared to a no-DMSO control.



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Description Size Supplier No. VWR Catalog Number Unit Price Quantity
P450-Glo™ CYP3A4 Assay with Luciferin-IPA 50 mL V9002 PAV9002 Each (50ml) Retrieving Restricted
P450-Glo™ CYP3A4 Assay with Luciferin-IPA 10 mL V9001 PAV9001 Each (10ml) Retrieving Restricted
restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
  • State issued document with your organization's Resale Tax ID Number
  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

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SPECIFICATIONS

Storage Conditions Store the CYP1A2, CYP2C9, and CYP3A4 membranes at –70 °C. Cytochrome P450 may lose activity with repeated freeze–thaw cycles. Avoid multiple freeze–thaw cycles by dispensing the CYP1A2, CYP2C9, and CYP3A4 membranes into single–use aliquots (e.g., 50 μL for 96 reactions). Store aliquots at –70 °C. All other components can be stored at –20 °C or –70 °C and away from light.
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