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SoftLink™ Soft Release Avidin Resin, Promega

Supplier: Promega Corporation
SoftLink Avidin Resin can be used for the isolation and purification of biotinylated molecules.

  • Item Binds 20-40 nmol of Biotinylated Protein per Milliliter of Resin
  • Bound Biotinylated Molecules Can Be Eluted under Mild Nondenaturing Conditions (5 mM Biotin)
  • Resin Retains Biotin-Binding Ability after Exposure to a Wide Range of pH, High or Low Ionic Strength, 6 m Guanidine, and 1% SDS
  • Purifications by Batch or Column Method
  • Item Supports High Flow Rates (300 cm/hour) and Centrifugal Forces (1500 x g) in Batch Applications

SoftLink Resin is a rigid, methacrylate polymeric gel filtration matrix functionalized with covalently bound, monomeric avidin. Monomeric avidin binds biotin wit Kd value of 10−7M, allowing reversible binding of bound biotinylated proteins under mild elution conditions.

Native, or tetrameric, avidin binds biotin with a very strong affinity (Kd = 10−15M), which in turn requires strong denaturing conditions for eluting bound material. Monomeric avidin allows the specificity of capture but also the mildness of release appropriate for the purification of sensitive biological materials.

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Description Size Supplier No. VWR Catalog Number Unit Price Quantity
SoftLink™ Soft Release Avidin Resin 5 mL V2012 PAV2012 Each (5ml) Retrieving

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

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Storage Conditions Store at 4 °C
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