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β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer, Promega

Supplier: Promega Corporation
The β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer is a convenient method for assaying β-galactosidase activity in lysates prepared from cells transfected with β-galactosidase reporter vectors such as the pSV-β-Galactosidase Control Vector.

  • For Measuring β-Galactosidase Enzyme Activity in Mammalian or Bacterial Cell Extracts
  • Non-Isotopic Assay
  • Assay Is Usable in a 96-Well Plate Format
  • Reporter Lysis Buffer Allows Firefly Luciferase, CAT, and β-Galactosidase Assays to Be Performed from the Same Cell Extract
  • System Has Sufficient Reagents for 65 Standard Assays or 200 Assays in a 96-Well Plate Format

The standard assay is performed by adding a dilute sample to an equal volume of Assay 2X Buffer that contains the substrate ONPG (o-nitrophenyl-β-d-galactopyranoside). Samples are incubated for at least 30 minutes while the β-Galactosidase hydrolyzes the colorless substrate to o-nitrophenyl, which is yellow.

The reaction can be terminated by addition of sodium carbonate, and spectrophotometry measures the absorbance at 420 nm. This system is appropriate for measuring β-galactosidase enzyme activity in mammalian or bacterial cell extracts.



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Description Size Wavelength Supplier No. VWR Catalog Number Unit Price Quantity
β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer 10 mL 420 nm E2000 PAE2000 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

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SPECIFICATIONS

Storage Conditions Reporter Lysis Buffer can be stored at room temperature. Store other system components at –20°C.
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