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Beta-Galactosidase Enzyme Assay System with Reporter Lysis Buffer, 10ml, Promega

Supplier: Promega Corporation
E2000
The Beta-Galactosidase Enzyme Assay System with Reporter Lysis Buffer is used to assay beta-galactosidase activity in cell lysates.

  • Colorimetric Detection of β-Gal Activity
  • Safe, non-isotopic assay
  • Can be used in 96-well plate format
  • Reporter Lysis Buffer allows luciferase, CAT and β-gal assays to be performed from the same cell extract

The Beta-Galactosidase Enzyme Assay System with Reporter Lysis Buffer is a convenient method for assaying beta-galactosidase activity in lysates prepared from cells transfected with beta-galactosidase reporter vectors such as the pSV-Beta-Galactosidase Control Vector. The standard assay is performed by adding a dilute sample to an equal volume of Assay 2X Buffer that contains the substrate ONPG (o-nitrophenyl-beta-D-galactopyranoside). Samples are incubated for at least 30 minutes, during which time the Beta-Galactosidase hydrolyzes the colorless substrate to o-nitrophenyl, which is yellow. The reaction can be terminated by addition of sodium carbonate, and the absorbance at 420mM is measured by spectrophotometry.

The reaction can be terminated by addition of sodium carbonate, and spectrophotometry measures the absorbance at 420 nm. This system is appropriate for measuring β-galactosidase enzyme activity in mammalian or bacterial cell extracts.

Samples are incubated for at least 30 minutes while the β-Galactosidase hydrolyzes the colorless substrate to o-nitrophenyl, which is yellow.




 

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Description Size Wavelength Supplier No. VWR Catalog Number Unit Price Quantity
Beta-Galactosidase Enzyme Assay System with Reporter Lysis Buffer, 10ml 10ml 420 nm E2000 PAE2000 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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SPECIFICATIONS

Storage Temperature -30°C to -10°C
Storage Conditions Reporter Lysis Buffer can be stored at room temperature. Store other system components at –20°C.
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