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AMPK (A1/B1/G1), Promega

Supplier: Promega Corporation
Recombinant full-length human AMPK (combination of A2/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag.

  • System Optimized for Quick Setup
  • Recombinant Kinase Enzyme Provided
  • Substrate Appropriate for the Enzyme Provided
  • Reaction Buffer and DTT Provided
  • Broad Dynamic Range for Less Ambiguous Data

AMPK (A2/B1/G1) plays a key role in insulin signaling pathways and is a major therapeutic target for the treatment of diabetes. AMPK is viewed as a fuel sensor for glucose and lipid metabolism by modulating the activity of the autonomous nervous system in vivo. Short-term overexpression of a constitutively active form of AMPK in the liver leads to mild hypoglycemia and fatty liver due to increased fatty acid utilization.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction. ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening and kinase-selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1 mM ATP.

Ordering information: Kinase Enzyme System contains Kinase [AMPK (A2/B1/G1), 10 ?g (Human, recombinant full-length), MW: ~69 kDa (A2); ~38 kDa (B1); ~40 kDa (G1)], substrate [SAMStide (HMRSAMSGLHLVKRR); derived from the mouse acetyl-Coenzyme A carboxylase alpha (amino acids 73–85)], reaction buffer, and DTT.

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Size Supplier No. VWR Catalog Number Unit Price Quantity
10 μg V1921 PAV1921 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

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To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
  • State issued document with your organization's Resale Tax ID Number
  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

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Storage Conditions Centrifuge the kinase and dispense it into smaller quantities as soon as it arrives. Store the kinase at –70 °C and the rest of the components at –20 °C.
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