Used as a standard cloning vector, a template for in vitro transcription or for production of circular ssDNA.
- Standard Cloning Vectors That Can Produce ssDNA and In vitro Transcribe Sense and Antisense RNA
- Blue/white screening for easy identification of recombinant clones
- In vitro transcription using the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
- The difference between + and - vectors is orientation of the f1 origin
The pGEM-3Zf(+) and pGEM-3Zf(-) Vectors are derived from the pGEM-3Z Vector and contain the origin of replication of the filamentous phage f1. These plasmids contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for EcoRI, SacI, KpnI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, SphI and HindIII. The pGEM-3Zf(+) and -3Zf(-) Vectors are identical except for the orientation of the f1 origin and can be used as standard cloning vectors, as templates for in vitro transcription and for the production of circular ssDNA.
The multiple cloning region contains unique restriction sites for EcoRI, SacI, KpnI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, SphI, and HindIII. The pGEM-3Zf(+) and -3Zf(–) Vectors are identical except for the orientation of the f1 origin and can be used as standard cloning vectors, as templates for in-vitro transcription, and for the production of circular ssDNA.
Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109).
