Taq DNA Polymerase + dNTPs in one kit.
- Contains dNTPs at 10mM
- Highly reproducible results
- Ideal for T/A cloning
- Ultra pure enzyme
- Highly processive
Taq CORE Kits contain all the reagents (presented as separate items) required for PCR: MP Biomedicals high quality recombinant Taq DNA polymerase, high purity dNTPs Mix (>99%), optimized incubation buffers with and without MgCl2 and a separate MgCl2 solution.
Applications: Nucleic Acid Amplification, PCR, RT-PCR, DNA sequencing and DNA labeling
Highly processive, recombinant, thermostable 5'-3' DNA polymerase catalyzes the addition of mononucleotide units to the 3'-OH end of a primer chain. Remains functional even after prolonged incubation at 95°C. This enzyme possesses 5'-3' exonucleases activity.
Quality control performed on every lot of enzymes: Specific PCR on genomic and phage templates, activity check, absence of ribonucleases, absence of nickases and endonucleases, absence of 3' exonucleases, purity on SDS-PAGE, absence of proteases.
Unit Definition: One unit is the amount of enzyme required to catalyze the incorporation of 10nmol of nucleotides into acid-insoluble material in 30min at 74°C under assay conditions.
Components: PCR reagents, Taq DNA polymerase, high purity dNTPs at 10mM each Mix (>99%), optimized incubation buffers with and without MgCl2 and a separate MgCl2 solution.
