Highly processive, recombinant, thermostable 5'-3' DNA polymerase.
- Highly reproducible results
- Ideal for T/A cloning
- Ultra pure enzyme
- Highly processive
DNA polymerase catalyzes the addition of mononucleotide units to the 3'-OH end of a primer chain
Quality control performed on every lot of enzymes: Specific PCR on genomic and phage templates, activity check, absence of ribonucleases, absence of nickases and endonucleases, absence of 3' exonucleases, purity on SDS-PAGE, absence of proteases.
Unit Definition: One unit is the amount of enzyme required to catalyze the incorporation of 10nmol of nucleotides into acid-insoluble material in 30 min at 74 °C under assay conditions.
Remains functional even after prolonged incubation at 95°C. This enzyme possesses 5'-3' exonucleases activity.
Components: 20mM Tris-HCL pH 8.0, 100mM KCl, 0.1 mM EDTA, 1mM dithiothreitol, 50% glycerol, Incubation buffer with MgCl₂, Incubation buffer without MgCl₂
