BBL™ Middlebrook and Cohn 7H10 Agar, BD

Supplier: BD Life Sciences
220958 297274 220959
90004-804PK 439.24 USD
90004-804 90001-162 90001-164
BBL™ Middlebrook and Cohn 7H10 Agar, BD
Media for Microbiology Prepared Media for Microbiology
Middlebrook and Cohn 7H10 Agar, when supplemented with Middlebrook OADC Enrichment, is used in qualitative procedures for the isolation and cultivation of mycobacteria.

  • Inorganic salts provides essential growth substances
  • Sodium citrate holds certain inorganic cations in solution
  • Glycerol provides carbon and energy
  • Malachite green dye partially inhibits bacterial contaminants

Supplementation of the agar base is required in order to obtain mycobacterial growth. In the enriched medium, sodium chloride maintains osmotic equilibrium; oleic acid, as well as other long chain fatty acids, can be utilized by tubercle bacilli and plays an important role in the metabolism of mycobacteria; the primary effect of albumin is that of protection of the tubercle bacilli against toxic agents and, therefore, it enhances their recovery on primary isolation; dextrose is an energy source; and catalase destroys toxic peroxides that may be present in the medium.
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