The ImProm-II Reverse Transcription System produces efficient, robust synthesis of first-strand cDNA in preparation for PCR amplification.
- Reverse Transcribe RNA Templates Up to 8.9kb
The ImProm-II Reverse Transcription System produces efficient, robust synthesis of first-strand cDNA in preparation for PCR amplification. The components of the ImProm-II Reverse Transcription System can be used to reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. The optimized reaction buffer and powerful ImProm-II Reverse Transcriptase provided in the ImProm-II System together enable robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The cDNA synthesis conditions were formulated for standalone applications or for easy transition to gene-specific target amplification. An aliquot of the reverse transcription reaction (1-20ul) can be amplified directly using Taq DNA polymerase in coupled or uncoupled PCR.
Ordering information: Each modifying enzyme is packaged in a tamper-evident, clear plastic, sealed packet that contains the enzyme tube, its buffer(s), and a lot-specific Promega Product Information (PPI) insert. ImProm-II reverse transcriptase system also comes with dNTP mix, oligo (dT)15 primer, random primers, 1.2 kb kanamycin positive control RNA, control primers, nuclease-free water, and recombinant RNasin ribonuclease inhibitor.
Caution: Storage temperature -30°C to -10°C
