RNase ONE™ Ribonuclease

Supplier: Promega
M4261 M4265
PAM4261EA 144.88 USD
PAM4261 PAM4265
RNase ONE™ Ribonuclease
Single-strand-specific RNA endonuclease. Cleaves between all ribonucleotides.

  • For sequencing and mapping
  • Degrades RNA to Cyclic Nucleotide Monophosphate (NMP) Intermediates
  • Ability to cleave a phosphodiester bond between any two ribonucleotides
  • Supplied at a concentration of 5-10u/microl

RNase ONE Ribonuclease is a 27kDa periplasmic enzyme from E. coli that catalyzes the degradation of RNA to cyclic nucleotide monophosphate (NMP) intermediates. Slower hydrolysis further catalyzes the degradation of these intermediates to 3'-NMPs. RNase ONE Ribonuclease is one of the few known RNases that can cleave a phosphodiester bond between any two ribonucleotides. RNase ONE Ribonuclease may be used to remove RNA from DNA preparations, for RNase protection assays and for mapping or quantitation of RNA by selective cleavage of single-stranded regions.
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