T4 DNA Ligase, PromegaSupplier: Promega
PAM1801EA 62.21 USDPAM1801 PAM1804
T4 DNA Ligase, Promega
Nucleic Acid Reagents Cloning Kits and Ligation
For ligation of DNA ends, mutagenesis and cDNA cloning.
- Joins Two DNA Strands with Cohesive or Blunt Ends
- Use with 5´, 3´ or blunt-ended dsDNA (e.g., inserts and vectors)
- Qualified for blue/white cloning, providing a higher level of quality control for enzymes used in cloning applications
- Can catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule
T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration. The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule but will not join single-stranded nucleic acids.
Caution: Storage conditions –30...–10 °C